Protein-protein interactions can be identified by a variety of techniques.

While not an all-inclusive list, several of the most common study types are listed below. 

BioID

 

Investigator provides affinity-enriched biotinylated proteins. MS Center performs digestion, TMT labeling, and peptide fractionation prior to MS analysis. Bioinformatic analyses are tailored for the confident identification of interacting partners. 


APEX

Investigator provides affinity-enriched biotinylated proteins. MS Center performs digestion, TMT labeling, and peptide fractionation prior to MS analysis. Bioinformatic analyses are tailored for the confident identification of interacting partners.


Epitope Tags

(HA, Flag, GFP, etc)

Investigator provides affinity-enriched proteins in buffer of known composition. MS Center performs digestion, TMT labeling, and peptide fractionationprior to MS analysis. Bioinformatic analyses are tailored for the confident identification of interacting partners. .