Our technologies support both peptide-level (e.g. bottom-up) and protein-level (e.g. top-down) analyses to identify and localize post-translational modifications. This includes, but is not limited to phosphorylation, ubiquitination, sumoylation, acetylation, glycosylation, and oxidative modifications. Depending on the goal of the study and modification type, specialized preparation workflows may be used to enrich for proteins or peptides with the modification of interest.

While not an all-inclusive list, several of the most common study types are listed below.

Phosphorylation

 

Investigator provides sample in a solution of known composition or in lyophilized form. MS Center performs enzymatic digestion and dual-chemistry enrichment (TiO2 & Fe-NTA) for phosphorylated peptides. Can be combined with quantitative workflows when appropriate.


Ubiquitination

Investigator provides sample in a solution of known composition or in lyophilized form. MS Center performs enzymatic digestion and identifies di-Gly modified lysines. Can be combined with quantitative workflows when appropriate.


Top Down Analyses

In "top down" analyses, intact proteins are analyzed directly (without pre-digestion) to detect degradation products, sequence variants, and combinations of post-translational modifications. This analysis maintains relationships of modifications throughout the length of the protein.